FAST FOOD CONSUMPTION AS A DRIVER OF GUT MICROBIOME REMODELING: MOLECULAR MECHANISMS, MICROBIAL DYSBIOSIS, AND CLINICAL IMPLICATIONS
Keywords:
Keywords: beta-diversity, intestinal microbiota, LC-MS profiling, microbial metabolites, NGS bioinformatics, qPCR expressionAbstract
Abstract. Frequent exposure to fast food rapidly alters intestinal community
structure and metabolic output, making coordinated molecular assessment necessary.
The aim was to determine how fast food intake relates to microbiota remodeling, short-
chain fatty acids, and clinical interpretation. A prospective cohort design was
implemented in N=192 participants using LC-MS, ecological diversity indices, NGS
bioinformatics, and qPCR. Acetate fell from 18.4±2.1 to 11.2±1.8 μmol/L, and butyrate
declined from 9.7±1.3 to 5.1±1.0 μmol/L; Shannon H' decreased from 3.21±0.18 to
2.64±0.16, while Margalef d shifted from 4.12±0.27 to 3.05±0.22. Prevotella increased
from 12.6±1.4 to 21.9±2.0 %, whereas Bacteroides dropped from 34.8±2.6 to 26.1±2.3
%; beta-diversity distance rose from 0.31±0.04 to 0.47±0.05, and qPCR log2FC moved
from 0.58±0.09 to -0.41±0.07, p=0.003. These concordant shifts support an integrated
biomarker framework for detecting diet-linked intestinal dysbiosis early.
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