GOSSIPOLNING AZO HOSILASI (NY-I) BILAN XROM(III) KOMPLEKS HOSIL QILISHINI SPEKTROFOTOMETRIK VA IQ-SPEKTROSKOPIK O'RGANISH
Keywords:
Kalit so'zlar: gossipol, azo hosilasi (NY-I), xrom(III), spektrofotometriya, IQ-spektroskopiya, kompleks hosil bo'lish, Buger–Lambert–Ber qonuni.Abstract
ANNOTATSIYA. Dolzarblik va maqsad. Atrof-muhit obyektlarida xrom birikmalarini nazorat qilish arzon va mahalliy xom ashyoga asoslangan analitik reagentlarni talab qiladi. Ishning maqsadi — gossipolning azo hosilasi (NY-I) bilan Cr(III) ionining rangli kompleks hosil qilish sharoitlarini aniqlash va ajratib olingan qattiq mahsulotni IQ-spektroskopiya yordamida tavsiflashdir. Usullar. UV-Vis spektrofotometriya (360–800 nm, l = 1,0 sm), pH-metriya va universal bufer eritmalar (pH 1,81–11,98) qo'llanildi; to'lqin uzunligi, pH, bufer turi, vaqt, komponentlarni qo'shish tartibi, reagent hajmi va xrom miqdorining ta'siri o'rganildi. Qattiq mahsulot NY-I va Cr(NO3)3 eritmalarini 50 °C da aralashtirib olindi va ATR-IQ usulida tahlil qilindi. Natijalar. Kompleks 485–500 nm sohada keng yutilish polosasini beradi (ish to'lqin uzunligi 492 nm). Optik zichlik pH 7,1 da maksimal; universal buferda ammiakli buferga nisbatan 7,4 baravar yuqori. Eritma 30 daqiqa barqaror; eng yuqori signal «reagent–xrom–bufer» tartibida olindi. Kalibrlash bog'lanishi 30–90 mkg/25 ml (1,2–3,6 mkg/ml) oralig'ida chiziqli (A = 0,00113·m + 0,059; R2 = 0,995; n = 3 nuqta), yuqorida to'yinish kuzatildi; ko'rinma molyar yutilish koeffitsienti (1,5–4,0)·103 l·mol−1·sm−1. IQ spektrda erkin ligandning 1702 sm−1 (aldegid C=O) polosasi yo'qoladi, 1613 sm−1 polosasi 1557 sm−1 ga siljiydi, O–H polosalari 3247 sm−1 da yagona keng polosaga birlashadi.
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